교수/실험실 실험실 소개 원예작물병리학 실험실 원예생명공학과
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교수/실험실

컨텐츠

원예작물병리학 실험실

서브 컨텐츠

주소 및 연락처
주소 및 연락처 - 주소, 연락처, 이메일, 홈페이지 제공표
주소 경기도 용인시 기흥구 덕영대로 경희대학교 생명과학대학 409호
연락처 031-201-2156
이메일 co35@khu.ac.kr
홈페이지 -
Research topics

1. Research on bacterial pathogenesis in Horticultural crops

(1) Elucidating virulence mechanisms of bacterial canker pathogens in tomato and pepper

Bacterial canker diseases in tomato and pepper are caused by Clavibacter michiganensis subsp. michiganensis and C. michiganensis subsp. capsici, which are Gram-positive plant-pathogenic bacteria (Figure 1). Although several virulence factors such as serine proteases and cellulase have been determined in these pathogens, pathogenesis mechanisms have not been well understood. Thus, we are trying to find critical virulence factors using diverse genomic analyses (Figure 2) and also to determine how these pathogens infect the host plants, tomato or pepper.

Figure 1. Pathogen morphology and disease sysmptoms in host plants

(USEM, 2016)

Figure 2. A comparison of the virulence regions between Cm ssp.
michiganensis strain NCPPB382 and Cm ssp. capsici strain PF008

(New Phytologist, 2017 in press).

2. Disease management and monitoring in Horticultural crops

(1) Developing phage therapy for bacterial canker in kiwifruit and fire blight in apple and pear

Bacterial canker in kiwifruit is very severe in most of kiwifruit-cultivating countries, including Korea, and is caused by Pseudomonas syringae pv. actinidiae, a Gram-negative plant-pathogenic bacterium. The fire blight is also very problematic worldwide and this disease was observed in Korea since 2015. To manage these diseases, antibiotics-based chemicals are somewhat effective. However, antibiotics-resistant bacterial strains have been emerged and also in many countries, including European countries and New Zealand, the use of antibiotics is prohibited. Therefore, to find alternative methods for managing these diseases are very urgent. Bacteriophages are the viruses killing only target host bacteria very specifically (Figure 3) and recently phage therapy using bacteriophages have been reconsidered as a biological control for bacterial diseases. We are trying to develop phage therapy to manage these bacterial canker and fire blight diseases in kiwifruit and apple and pear trees.

Figure 3. Bacteriophages effective to bacterial canker in kiwifruit

(JMB, 2016)

(2) Monitoring for fire blight and black shoot blight in apple and pear

Fire blight disease caused by Erwinia amylovora was reported in Korea in 2015 (Figure 4). In addition, bacterial black shoot blight disease is very similar to fire blight disease in apple and pear. This disease was first reported in Korea and is caused by Erwinia pyrifoliae, a Gram-negative plant-pathogenic bacterium. Nationwide extensive eradication programs have been operated in Korea for these diseases. However, ecology of these diseases or pathogens has not been well studied. Now, we are trying to monitor these diseases in apple and pear orchards in Korea.

Figure 4. Symptoms of fire blight diseases in asian pear. (A) Blighted flower.
(B & C) Blighted young shoots. (D) Blighted branch

(plant Disease, 2016)

3. Research on plant immunity in horticultural crops

(1) Examining disease resistance to bacterial wilt and bacterial canker in tomato

First, bacterial wilt disease in tomato is caused by Ralstonia solanacearum, which is a Gram-negative plant-pathogenic bacterium. This pathogen can survive in soil for a long time. Once the host plant, tomato, is planted, a pathogen infects the plant through roots and it enters into xylem tissue eventually. Then, bacterial pathogens multiply and block water movement mostly by exopolysaccharides (EPS). Second, bacterial canker disease in tomato is caused by Clavibacter michiganensis subsp. michiganensis, which is a Gram-positive plant-pathogenic bacterium. This bacterium also infects xylem tissue and is transmitted by seeds. So far, not much tomato genetic resources to carry disease resistance against each disease have been reported. We are collecting tomato genetic resources to carry disease resistance against each disease and are aiming to study genetic factors to determine disease resistance to bacterial wilt or bacterial canker disease in tomato, using collected genetic resources.

(2) Examining mechanism of hypersensitive response (or programmed cell death) mediated by plant immunity in tomato and tobacco

Plants have molecular mechanisms to recognize and trigger disease resistance to diverse pathogens. In particular, bacterial plant pathogens carry two different types of elicitors: PAMPs (pathogen-associated molecular patterns) such as flagellin and peptidoglycan and effector proteins. Effector proteins are proteins that are secreted from pathogens and delivered into plant cytoplasm. Mostly, they act as virulence factors. Certain plant cultivars have resistance (R) proteins to recognize corresponding effector proteins and trigger effector-triggered immunity (ETI). Once ETI is triggered, the hypersensitive response (HR), which is a localized and rapid cell death at the infection sites, is followed. Although Clavibacter michiganensis subsp. michiganensis does not carry typical effectors, it can induce HR in Nicotiana benthamiana. We are examining pathogen factors carrying HR-eliciting activity and also their mechanisms to cause HR in this plant.

연구진 소개
연구진 소개 목록
  • 연구진명 : 황인선
  • 직위 : 학술연구교수
  • 연구실명 : 원예작물병리학 실험실
  • 이메일 : -
  • 소속기관 : -
  • 학사취득학교 : -
  • 소속부서 : -
  • 최종학위 및 취득학교명 : 고려대학교 박사
  • 홈페이지 : -
  • 연구진명 : Roniya Thapa Magar
  • 직위 : Post-doctoral Researcher
  • 연구실명 : 원예작물병리학 실험실
  • 이메일 : roniyathapa@gmail.com
  • 소속기관 : -
  • 학사취득학교 : Tribhuvan University, Nepal
  • 소속부서 : -
  • 최종학위 및 취득학교명 : Ph.D., Dong-A University, South Korea
  • 홈페이지 : -
  • 연구진명 : 오엄지
  • 직위 : 박사과정
  • 연구실명 : 원예작물병리학 실험실
  • 이메일 : -
  • 소속기관 : -
  • 학사취득학교 : -
  • 소속부서 : -
  • 최종학위 및 취득학교명 : -
  • 홈페이지 : -
  • 연구진명 : 송유림
  • 직위 : 박사과정
  • 연구실명 : 원예작물병리학 실험실
  • 이메일 : -
  • 소속기관 : -
  • 학사취득학교 : -
  • 소속부서 : -
  • 최종학위 및 취득학교명 : -
  • 홈페이지 : -
  • 연구진명 : 박인웅
  • 직위 : 박사과정
  • 연구실명 : 원예작물병리학 실험실
  • 이메일 : -
  • 소속기관 : -
  • 학사취득학교 : -
  • 소속부서 : -
  • 최종학위 및 취득학교명 : -
  • 홈페이지 : -
  • 연구진명 : VU TRUNG NGUYEN
  • 직위 : 석박사통합과정
  • 연구실명 : 원예작물병리학 실험실
  • 이메일 : -
  • 소속기관 : -
  • 학사취득학교 : -
  • 소속부서 : -
  • 최종학위 및 취득학교명 : -
  • 홈페이지 : -
  • 연구진명 : NGUTEN THI THUONG
  • 직위 : 석박사통합과정
  • 연구실명 : 원예작물병리학 실험실
  • 이메일 : -
  • 소속기관 : -
  • 학사취득학교 : -
  • 소속부서 : -
  • 최종학위 및 취득학교명 : -
  • 홈페이지 : -
  • 연구진명 : 김요엘
  • 직위 : 석사과정
  • 연구실명 : 원예작물병리학 실험실
  • 이메일 : -
  • 소속기관 : -
  • 학사취득학교 : -
  • 소속부서 : -
  • 최종학위 및 취득학교명 : -
  • 홈페이지 : -

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